Warner PC-505B - OPERATION; Core Operation Procedures; THEORETICAL CONSIDERATIONS; Electrode and Reference Electrode Topics

PC-505B Manual, Rev. 201013
Cap Comp checkout............................................................................................................................ 24
Current clamp checkout ..................................................................................................................... 24
OPERATION ............................................................................................................................................ 26
Initial settings ........................................................................................................................................ 26
Preliminary ........................................................................................................................................... 27
Mount the electrode ............................................................................................................................ 27
Submerge the electrode tip ................................................................................................................. 27
Zero the pipette offset ......................................................................................................................... 27
Measure the pipette resistance (RP)................................................................................................... 28
Voltage clamp........................................................................................................................................ 29
Attach to the cell and form gigaseal ................................................................................................... 29
Set electrode capacitance compensation ............................................................................................ 30
Measure seal resistance (RS) ............................................................................................................. 30
Patch or whole cell recording? .......................................................................................................... 31
Patch recording - Leak subtraction .................................................................................................... 31
Patch recording – Excised patch ........................................................................................................ 31
Patch recording - Cell-attached patch ............................................................................................... 32
Whole-cell recording – Initial conditions........................................................................................... 32
Whole-cell recording - Rupture the membrane patch ........................................................................ 32
Whole-cell recording - Series R compensation .................................................................................. 33
Whole-cell recording - % correction .................................................................................................. 34
Current clamp ....................................................................................................................................... 35
Switching into current clamp ............................................................................................................. 35
Recording in current clamp ................................................................................................................ 35
External command currents ............................................................................................................... 36
Bilayer recording .................................................................................................................................. 36
THEORETICAL CONSIDERATIONS ................................................................................................. 37
Chloriding silver wire ........................................................................................................................... 37
Chloriding by electrolysis .................................................................................................................. 37
Chloriding chemically ........................................................................................................................ 37
Electrode holders .................................................................................................................................. 37
Care and use of holders...................................................................................................................... 38
Cleaning and storage ......................................................................................................................... 38
Replacing holder parts ....................................................................................................................... 38
Reference electrodes ............................................................................................................................. 39
APPENDIX ................................................................................................................................................ 40
Specifications......................................................................................................................................... 40
Accessories and replacement parts ..................................................................................................... 42
Warranty and service ........................................................................................................................... 43
Warranty ............................................................................................................................................. 43
Service notes ....................................................................................................................................... 43