10. Set GAIN to 10 mV/pA.
11. Verify that oscilloscope reads +1 V (1 div at these settings)
12. Set VOLTAGE HOLD to negative (–).
13. Verify that METER reads –10 pA.
14. Verify that oscilloscope reads –1 V (1 div at these settings)
15. Return amplifier to initial settings
Cap Comp checkout
1. Connect model cell to headstage in patch mode.
2. Set PROBE RESISTOR to high
3. Set VOLTAGE HOLD control to off.
4. Set scope to 5 V/div.
5. Set COMMAND SELECT to internal command.
6. Set COMMAND SENSITIVITY toggle to on.
7. Scope should now display large amplitude positive and negative pulses. Clipping LED
will be lit.
8. Using FAST CAP COMP controls, minimize amplitude and duration of pulses. (Leading edge
transients will remain due to characteristics of model cell.)
9. Set PROBE RESISTER to low.
10. Connect model cell to headstage in whole cell mode.
11. Turn C–SLOW toggle switch on.
12. Adjust C–SLOW and SERIES R controls until leading edge of square wave transitions
smoothly from one peak to the other. Full transition should take approximately 1 ms.
Remaining leading edge transients can be minimized by small adjustments to the FAST
CAP COMP controls.
13. Turn % CORRECTION toggle switch on. Verify that control decreases transition time
without increasing amplitude of spike.
14. Turn % CORRECTION off.
15. Turn LEAK SUBTRACTION on.
16. Verify that control adjusts amplitude of square wave.
17. Remove model cell from headstage.
18. Return amplifier to initial settings.
Current clamp checkout
1. Attach model cell to headstage in bath mode.
2. Set MODE toggle to I
o.
3. Set METER SELECT to V
m
.
4. Using JUNCTION ZERO control adjust METER to 0 mV.
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