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Warner PC-505B - Whole-Cell Recording: % Correction; % Correction Optimization

Warner PC-505B
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PC-505B Manual, Rev. 201013
Warner Instruments
A Harvard Apparatus Company
34
Return to SERIES R and adjust the overshoot
away. Go back and forth between the C-SLOW
and SERIES R controls until you’ve completely
removed the leading spike and made the
leading corner as sharp as possible. An
example is shown to the right.
The value of Series R can now be read directly from
the SERIES R dial.
Whole-cell recording - % correction
Once the series resistance has been measured and corrected, you can further optimize
the amplifier by applying a signal boost in the form of % correction. Generally speaking, the
maximum correction that can reasonable be applied is 80%.
Activate the % CORRECTION control and raise its
value to 5% or so. Doing so will distort the
previously Series R settings, so you will need to
lower C-SLOW to compensate. Gradually go back
and forth, increasing % CORRECTION and decreasing
C-SLOW until you have maximized the sharpness of
the leading corner on the trace without ringing. An
example is shown to the right.

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