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Warner PC-505B User Manual

Warner PC-505B
51 pages
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PC-505B Manual, Rev. 201013
Warner Instruments
Whole Cell/Patch Clamp Amplifier
Model PC-505B
Warner Instruments
1125 Dixwell Avenue, Hamden, CT 06514
(800) 232-2380 / (800) 547-6766 - support
www.warneronline.com

Table of Contents

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Warner PC-505B Specifications

General IconGeneral
BrandWarner
ModelPC-505B
CategoryAmplifier
LanguageEnglish

Summary

INTRODUCTION

Nomenclature and Conventions

Defines terms, abbreviations, and signal polarity conventions.

Control Description - Front Panel

Details the controls on the instrument's front panel.

SETUP AND INITIAL TEST

Headstage Handling and Preparation

Precautions and steps for preparing the headstage for use.

Test Procedures Overview

Outlines the sequence of tests for instrument checkout.

OPERATION

Core Operation Procedures

Covers initial settings, voltage/current clamp, and bilayer recording steps.

THEORETICAL CONSIDERATIONS

Electrode and Reference Electrode Topics

Discusses electrode preparation, handling, and reference electrodes.

APPENDIX

Specifications, Parts, and Service

Lists specs, parts, warranty, and service information.

NOMENCLATURE

Text Conventions

Explains manual formatting for clarity and consistency.

Device Panel Abbreviations

Lists and defines abbreviations for instrument controls and indicators.

Signal Polarity Conventions

Defines positive and negative conventions for membrane current and potential.

CONTROL DESCRIPTION

Front Panel Controls Overview

Describes the organization and purpose of front panel control blocks.

Control Description - Commands Block

Test Pulse Functionality

Explains the generation and use of the internal test pulse.

Junction Zero and Auto Zero Controls

Details controls for setting zero baseline and compensating offsets.

Control Description - Zap and Compensation

Zap Function for Membrane Penetration

Describes the ZAP function for membrane penetration.

Fast Capacitance Compensation

Details controls for fast capacitance compensation.

Whole Cell Command Block Controls

Describes controls for whole cell recording compensation.

Control Description - Output and Compensation

Series R and % Correction

Explains compensation for series resistance and signal boost.

Leak Subtraction

Explains compensation for leakage currents.

Output Command Block and Probe Resistor Select

Details output modes and headstage resistor selection.

Control Description - Modes and Filtering

Clamp Mode Selection

Explains the different clamp modes: voltage, zero current, and current.

Gain and Filter Settings

Details gain selection and the 4-pole Bessel filter.

Voltammetry Mode

Explains the instrument's function as a potentiostat.

METER Section and Outputs

Meter Selections and Readings

Describes the different meter readings for voltage, current, and noise.

Front Panel BNC Connectors

Details the output BNC connectors on the front panel.

Rear Panel Connectors and Signals

Rear Panel BNC Inputs/Outputs

Describes rear panel BNC connectors and their signals.

Gain Telegraph Output

Explains the gain telegraph signal and its settings.

Rear Panel Interface and Controls

Filter Telegraph and Sync Out

Describes filter setting telegraph and synchronization output.

Rear Panel Connectors and Switches

Details headstage connection, PSTAT, and SPEED TEST controls.

Grounding and Headstage Information

Ground Connections

Discusses circuit and chassis ground connections for noise reduction.

Headstage Overview and Types

Describes the headstage's function and lists different models.

Electrode Holders and Model Cell

Electrode Holder Details

Provides details on electrode holder types and usage.

Model Cell Description

Describes the model cell used for familiarization and troubleshooting.

SETUP AND INITIAL TEST

Power and Grounding

Requirements for line voltage and instrument grounding.

Headstage Handling Precautions

Crucial safety precautions for handling the static-sensitive headstage.

Headstage Preparation and Test Procedures

Headstage Preparation Steps

Steps to prepare the headstage, including grounding and connections.

Test Procedures Setup

Lists tools and initial connections required for instrument checkout.

Initial Instrument Configuration

Front Panel Control Settings

Specifies initial settings for front panel controls.

Oscilloscope Setup Parameters

Specifies initial settings for oscilloscope parameters.

Instrument Checkout Procedures

RMS Noise and Im Output Checks

Procedures for checking RMS noise and Im output.

Junction Zero and Internal DC Voltage Command Verification

Procedures to verify Junction Zero and internal DC voltage commands.

Checkout Procedures - Voltage Commands

External DC Voltage Command Verification

Procedure to check external DC voltage commands.

Checkout Procedures - AC, Auto Zero, and Vc Commands

Internal AC Voltage and Auto Zero Verification

Procedures to check internal AC voltage and Auto Zero controls.

Vc Commands Verification

Procedure to check Vc commands.

Checkout Procedures - Compensation and Current Clamp

Capacitance Compensation Checkout

Procedure to check capacitance compensation controls.

Current Clamp Checkout

Procedure to verify current clamp mode operation.

OPERATION

Initial Settings Configuration

Lists initial settings for amplifier controls before operation.

Preliminary Steps

Electrode Mounting and Submerging

Steps to mount and submerge the electrode tip in solution.

Pipette Offset Zeroing

Procedure to establish a zero baseline reference potential.

Pipette Resistance Measurement

Measuring Pipette Resistance (RP)

Method for determining pipette resistance before recording.

Voltage Clamp Operation

Cell Attachment and Gigaseal Formation

Steps to attach to the cell and form a gigaseal.

Voltage Clamp: Compensation and Seal Measurement

Electrode Capacitance Compensation

Procedure for compensating electrode capacitance.

Seal Resistance (RS) Measurement

Method for measuring seal resistance (RS).

Patch vs. Whole-Cell Recording

Patch Recording: Leak Subtraction

Compensating for membrane voltage errors due to leak.

Patch Recording: Excised Patch

Procedure for excising a membrane patch from the cell.

Patch Recording Techniques

Cell-Attached Patch Recording

Procedure for performing cell-attached patch recordings.

Whole-Cell Recording

Initial Conditions and Membrane Rupture

Setting initial conditions and rupturing the membrane patch.

Whole-Cell Recording: Series R Compensation

Series R Compensation Procedure

Compensating for series resistance using SERIES R and C-SLOW controls.

Whole-Cell Recording: % Correction

% Correction Optimization

Optimizing amplifier performance using % correction.

Current Clamp Operation

Switching to Current Clamp Mode

Steps to switch the amplifier to current clamp mode.

Performing Current Clamp Recordings

Guidelines for recording in current clamp mode.

Current Clamp and Bilayer Recording

External Command Current Capabilities

How external commands influence current capability.

Bilayer Recording Procedures

Guidelines for performing bilayer recordings with the HB-205B headstage.

THEORETICAL CONSIDERATIONS

Silver Wire Chloriding Methods

Explains methods for chloriding silver wire for electrodes.

Electrode Holder and Reference Electrode Details

Information on electrode holders and reference electrodes.

Electrode Holder Maintenance

Care, Cleaning, and Part Replacement

Guidelines for caring for, cleaning, and replacing parts of electrode holders.

Reference Electrode Functionality

Reference Electrode Details and Usage

Detailed explanation of reference electrodes and their use.

APPENDIX

Instrument Specifications

Lists detailed specifications for headstages, voltage/current clamp commands, and compensation.

Accessories and Replacement Parts

Lists available accessories and replacement parts for the instrument.

Warranty and Service Information

Details the product warranty and service procedures.

Output Signals and Operating Parameters

Front and Rear Panel Outputs

Describes the signals available at front and rear panel outputs.

Operating Conditions and Physical Dimensions

Specifies operating environment and physical dimensions.

Accessories and Replacement Parts

Headstage Models and Electrode Holders

Lists headstage models and electrode holder types with part numbers.

Warranty and Service

Warranty Coverage and Limitations

Details the product warranty coverage and limitations.

Service Notes and Procedures

Provides notes on common service issues and procedures.

Certifications

Declaration of Conformity (EMC)

Declaration of conformity to EU directives for EMC.

Declaration of Conformity (LVD)

Application of Council Directive 73/23/EEC

Declaration of conformity to EU directives for LVD.

EU Directives Compliance

WEEE/RoHS Compliance Statement

Statement on compliance with WEEE and RoHS directives.

Glossary of Terms

A/D Converter, Analog, and Bandwidth

Definitions for A/D converter, analog, and bandwidth.

Bessel Filter, BNC Connector, and Capacitance Terms

Definitions for Bessel filter, BNC connector, capacitance, and compensation.

Command Input and Sensitivity Terms

Definitions for CMD IN, command sensitivity, and command voltage.

Glossary Continued

Faraday Cage, Gain, and Ground Loop

Definitions for Faraday cage, gain, gain telegraph, and ground loop.

Headstage and Im Definition

Definitions for headstage and Im (membrane current).

Glossary Continued

Junction Potential, LED, and Mini-Jack

Definitions for junction potential, LED, mini-jack, and related terms.

Model Cell, Oscilloscope, and Signal Polarity

Definitions for model cell, oscilloscope, output, signal polarity, and time constant.

Potentiometer and Transient Terms

Definitions for potentiometer, transient, transmembrane, and trim pot.

Glossary Continued

TTL, Unitary Channel Conductance, and Vm HOLD

Definitions for TTL, unitary channel conductance, and Vm HOLD.

Vc Definition

Definition for Vc.