WPI EVOM3 - Testing STX2-PLUS Electrodes

28 World Precision Instruments
Testing the STX2-PLUS Electrodes
If another electrode is being used, refer to that electrode's manual.
To make sure the electrode is operating properly, a resistance test in KCl is used. This
test demonstrates electrode functionality.
20 mM 40 mM 80 mM 160 mM
 Ω  Ω  Ω  Ω
At the lower molarities (10–20 mM), a DMEM coated electrode will begin to show
instabilities at the higher concentrations. If the resistance readings in the KCl mixtures
are over 10% out of range, then the electrode may need cleaning. See "Cleaning/
Maintaining the STX2-PLUS Electrodes" on page 26. An Excel scatter plot of the
molarity vs. resistance (as a power) should show an R
2
value > 0.99x.
 hee eitane eading an a de t id lme and eletde deth
These values are a reference. An Excel scatter plot of the molarity vs. resistance
(as a power) should show an R
2
value > 0.99x. If the R
2
value is acceptable, then the
electrode can be used even if it is slightly out of range. If the resistance readings in
the KCl mixtures are over 10% out of range, then the electrode may need cleaning.
See "Cleaning/Maintaining the STX2-PLUS Electrodes" on page 26. At the lower
molarities (20 mM), a DMEM coated electrode may begin to show instabilities
indicating a need for cleaning.
ACCESSORIES
WPI Part # Description
EVOM3 ACCESSORIES & REPLACEMENT PARTS
99672 EVOM3 legacy electrode Adaptor to use EVOM3 with EVOM2 electrode
99673 EVOM3 aliatin Kit  Ω  et Reit
99675 EVOM2 Electrode adaptor to use EVOM2 with EVOM3 electrodes
99916 EVOM3 EndOhm cable
803026 EVOM3 upgrade cable USB Mini-B
300749 USB drive 32GB, programmed
503535 USB2 cable
803025 EVOM3 A/C mains power supply and battery charger
803028 USB Drive, 32 Gb (Western Digital Sandisk # SDCZ60-032G-A46 - Factory
tested for use with the EVOM3)
13142 Footswitch
3993 2 mm Adapter for EVOM2
ELECTRODES FOR TEER (EPITHELIAL) MEASUREMENT
STX2-PLUS Replacement Electrode Set
STX2 Relaement letde et (Reie 99672 for use with the EVOM3)
STX3 dtale letde et  hall ell,  mm deth (Reie
99672 for use with the EVOM3)
3993 2 mm Adapter for EVOM2
ENDOHM CHAMBERS FOR ENDOTHELIAL/EPITHELIAL MEASUREMENT
ENDOHM-6G EndOhm for 6 mm culture cup (24 wells per plate)