WPI EVOM3 - TEER Calculations

EVOM3
World Precision Instruments 43
The larger the membrane, the lower the resistance. The dimension is Ω-cm
2
, not Ω /
cm
2
.
TEER Calculations
R i a nmalied ale  eitane e  ae entimete  nit aea 
compute TEER, multiply the measured resistance by the surface area’s listed below.
F eamle, a  mm inet meae  Ω, the R i  Ω     Ω
• 6 well plate (24 mm inserts) 4.53 cm
2
• 12 well plate (12 mm inserts) 1.13 cm
2
• 24 well plate 6.5 mm inserts) 0.3316 cm
2
• 96 well plate (4.3 mm inserts) 0.145 cm
2
APPENDIX G: FREQUENTLY ASKED QUESTIONS
1. Will the EVOM3 work with the EndOhms?
Answer: Yes, but an 99916 adat i eied ( a ne ale)
2. When do researchers want to use the blank function?
Answer: The blank function allows researchers to subtract out resistance
measurement that is not caused by the membrane. This could be a reading
aeted  the eletde  id eitane
3. Does the system automatically calculate TEER?
ne  allating R eie an aea allatin ee R allatin
on page 2 and "Appendix F: Resistance Calculations" on page 41.)
4. The program is automatically stored when the last well is reached. What happens
when I only want to measure 8 of 96 wells and store the program?
Answer: Once you’re done measuring, open the settings, press the store screen
menu, then store the plate data to the USB.