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WPI EVOM3 - TEER Calculations

WPI EVOM3
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2 World Precision Instruments
Select
Filename
to enter a new name.
Press
Clear
.
Use the on screen keypad to enter a new name.
Press
Enter
.
NOTE t indeing hange the name t a nmei eene matted ith
the name, a efi and a eential nme (nameefi)
3. Press
Setup
to access the
Setup
menu.
Select Mode–Set the mode to PD.
Select Plate–Choose the plate size.
Display Units– Select the display units in volts or millivolts. (The normal mode
is millivolts.)
Calibrate (zero) the electrodes in saline. Press the
Probe Null
button. Push the
Null
button. Wait for the second message stating ”Solution value 0.0” before
etning hi atin eme an ltage et n the eletde
Press
Return
to navigate back to the main menu.
4. Insert a USB thumb drive into the USB port on the side of the EVOM3 and begin
measuring by pressing the foot switch or touching the
Store
button on the screen.
Tap the
Next Well
area on the right side of the screen to advance to the next well.
Repeat the sampling process.
 ne the lat ell ha een eded, the file ting eletin ntifiatin
appears. Press
Store
. A system message will report if the USB thumb drive is
usable, or it will display an error. If the USB thumb drive is not plugged in or it
is not recognized, a warning message will appear. Press
Cancel
and try another
drive. The
Store
function cannot be used on an unrecognized drive. EVOM3
hld end ith File aed ithin  end  aing file i till een
after 20 seconds, press the
Return
e and e anthe  die ne a file ha
een aed, en the file n a mte t ei the ntent
NOTE he file aing tine an tae  t  end t mlete ee the
USB cautions on page 5.
 Reme the  thm die t tane the data file t  mte a a V
file (el)
TEER Calculations
To compute TEER, multiply the measured resistance by the surface area’s listed below.
F eamle, a  mm inet meae  Ω, the R i Ω     Ω
6 well plate (24 mm inserts) 4.52 cm
2
12 well plate (12 mm inserts) 1.13 cm
2
24 well plate 6.5 mm inserts) 0.3316 cm
2
96 well plate (4.3 mm inserts) 0.145 cm
2

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