XCell ATF Series - Screen Module Replacement; Diaphragm Replacement; Connection to Bioreactor

52
XCell
TM
ATF System with C410:V3 Controller
User Guide
Step 2 is to avoid damage to the “O” ring during its insertion and sliding into the
housing, to avoid crossing the Harvest port opening during insertion, as the Drain
port is close to the housing end.
2 Expose HFM at the Filter Housing end distant to Drain port, in proximity to the
Filtrate / Harvest port.
3 Place an “O” ring in the exposed HFM “O” ring groove.
4 Press the HFM with “O” ring firmly into the housing.
Assure the “O” ring is inserted smoothly and uniformly. Lubricate with WFI to
facilitate this process.
5 Press until the HFM “O” ring groove is exposed at the other end, the Drain
nozzle end.
6 Place 2
nd
“O” ring on the exposed end.
7 Press the HFM back into the Filter Housing until both “O” rings sealed against
the housing ID.
8 Assemble the system.
7.3.2 I-Line HFM insertion:
1 Stand the Filter Housing vertically, with harvest port up.
2 Insert the HFM into the housing
3 Insert one “O” ring into the groove formed between HFM and Filter Housing
end-ferrule wall.
4 Place the reducer onto the end, forcing the “O” ring into the groove.
5 Clamp the reducer to the housing, the compressed “O” ring is forced against the
HFM. A seal is formed between the Housing, Reducer and HFM
6 On the other end of the Filter Housing, Place the second “O” ring into the
formed groove between HFM and Housing end-ferrule.
7 Carefully place the Filter Housing onto the Diaphragm Pump, forcing the “O”
ring deeper into the groove.
8 Clamp the Filter Housing to the Diaphragm Pump.
9 Assemble the XCell
TM
ATF System.
7.4 Screen Module replacement:
If the SM is being used, contact Repligen for replacement instructions.
7.5 Diaphragm replacement:
Replacement or placement of a diaphragm within the Diaphragm Pump is part of the
Diaphragm Pump assembly process which differs slightly among the XCell
TM
ATF4
System, ATF6 System, and ATF10 System.
Diaphragm Replacement Procedure for the XCell
TM
ATF4 System and ATF6 System
1 Place cleaned Diaphragm Pump hemispheres, diaphragm and pump clamp on a
table top