XCell
TM
ATF System with C410:V3 Controller
9.1 Hard Connection
The user should verify the following procedure:
1 Typically used with stainless steel bioreactor systems where steam is readily
available.
2 This connection consists of at least two isolation bivalves (Figure 1). One bivalve
attached to the vessel and the second bivalve attached to the Filtration Assembly.
3 A flexible hose between the main branches of the two valves.
4 Steam inlet- The side valve on one of the bivalves may be used for steam inlet.
5 Condensate-The side valve on the other bivalve is used for drain condensate. This
valve should be the low point valve to assure complete condensate drainage. Attach
a steam trap to this valve and connect to a drain
6 Close both Steam inlet and Condensate valves.
7 Securely connect a regulated steam source to the steam inlet valve.
8 Open steam service.
9 Slowly open steam inlet valve.
10 Slowly open steam condensate valve.
11 Sterilize connection for about 20min. at 121
0
C and ~16psi
12 Cool-down.
13 Close the Condensate valve.
14 Rapidly close the Steam inlet valve and immediately open the main valve into the
Filtration Assembly to minimize a pressure drop in the connection.
Note that following SIP of the connection, the cool-down of the connection post SIP
will generate a vacuum within the connection, which must be minimized.
10. Maintenance
10.1 Diaphragm
It is recommended that a new diaphragm be used with each new run. To avoid
premature failure, it is important to ensure that the diaphragm is seated in the proper
orientation.
Use with aqueous liquids only. For use with other fluids contact Repligen.
When used as instructed in this manual, the diaphragm is designed to last over 500,000
cycles.
10.2 Pump Air Inlet Filter
The replacement of the pump air inlet filter will be determined by how the system is
configured. If the air filter is autoclaved with the Filtration Assembly, it is qualified for
135°C, for 30minutes, 3 times.
10.3 “O” Rings, gaskets and Quick Connects
The o-rings on the ventilation ports and quick connects (XCell
TM
ATF4 System and ATF6
System) should be replaced every two or three runs. Replacement kits are available from
Repligen. HFM Filter “O” rings should be replaced with each new filter. For pump line,
air supply, and vacuum source, all “O” rings and gaskets should be replaced periodically.
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