2-7
To install a membrane, first unscrew the appropriate probe retainer and gently pull the probe
out of the block. Remove the existing O-ring membrane assembly from the end of the probe. A
toothpick or pointed tool may be needed to unseat the old membrane. Be careful not to scratch
the probe face.
Examine the probe surface and remove any pieces of membrane that remained. Open a cavity of
the plastic membrane holder and rinse the membrane inside with a few drops of salt solution
(YSI 2392 or equivalent). Place one drop of salt solution on the probe face. Using the plastic
membrane holder, press the O-ring membrane assembly gently onto the probe face (Figure 2.5).
Wipe excess salt solution from the probe body, then return the probe to the sample chamber.
Finger tighten the probe retainer so that the O-ring seals the probe in place. Do not overtighten.
Repeat this procedure for the second probe if you have a dual channel unit.
Return the membrane holder to the foil pouch and refrigerate it. Note the expiration date on the
membrane package. It is advisable to maintain an instrument log book in which dates and lot
numbers of reagents are recorded, along with information from daily operational checks and
other relevant information (see Section 3.3 for more information).
Figure 2.5
Enzyme Membrane Installation
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