YSI 2700 SELECT - 4.5 L-Lactate

4-6
" ##
This is a direct reading of L-Lactate (L-Lactic Acid) in solution at the enzyme sensor. The
enzyme L-Lactate Oxidase is immobilized in the YSI L-Lactate Membrane.
L-Lactate + O
2
L-Lac Oxidase
H
2
O
2
+ Pyruvate
System Buffer YSI 2357
Calibrator Std YSI 2776 (0.50 g/L L-Lactate)
Linearity Std YSI 1530 (2.67 g/L L-Lactate)
Black Probe YSI 2329 L-Lactate Membrane
White Probe n/a *
Sample Size 25 µL
Cal Station Station #1 Cal Well
Black Probe Parameters
Chemistry L-Lactate
Unit of Conc g/L
Cal value 0.50
End Point 30 sec
* If you have a dual channel unit, either Black or White, or both Black and White probes can be
configured for this chemistry.
Note: See Appendix A if concentration unit conversion is required.
Special Considerations:
»
If sample dilution is required, use reagent water or 2357 buffer.
»
D-Lactate is not a substrate for L-Lactate Oxidase.
Therefore, the 2700 SELECT
cannot directly measure D-Lactate. If you have a known racemic mixture of lactates,
the L-Lactate value multiplied by 2 should give you the total lactate value.