4-15
#
This is a direct reading of glutamine in solution at the enzyme sensor. Two enzymes are co-
immobilized in the YSI Glutamine Membrane: Glutaminase and Glutamate Oxidase.
L-Glutamine
Glutaminase
L-Glutamate + NH
3
L-Glutamate + O
2
Glut Oxidase
H
2
O
2
+ α-Ketoglutarate + NH
3
Through this chain of reactions the amount of hydrogen peroxide liberated is directly
proportional to the amount of glutamine.
System Buffer YSI 2357
Calibrator Std YSI 2736 (5.00 mmol/L)
Linearity Std. YSI 2737 (8.00 mmol/L)
Black Probe YSI 2735 Glutamine Membrane
White Probe n/a *
Sample Size 20 µL
Cal Station Station #2
Black Probe Parameters
Chemistry Glutamine
Unit of Conc mmol/L
Cal value 5.00
End Point 30 sec
* If you have a dual channel unit, either Black or White, or both Black and White probes can be
configured for this chemistry.
Note: See Appendix A if concentration unit conversion is required.
Special Considerations:
»
If sample dilution is required, use reagent water or 2357 buffer.
The sample must be
glutamate-free
, or at least contain levels low enough not to interfere with
the glutamine reading. Since the glutamine membrane contains glutamate oxidase, glutamate
will produce a probe signal. See Section 4.17 to measure glutamate and glutamine
simultaneously.
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