4-8
% #
This is a direct reading of lactose in solution at the enzyme sensor. The enzyme Galactose
oxidase is immobilized in the enzyme membrane.
Lactose + O
2
Gal Oxidase
H
2
O
2
+ Galactose Dialdehyde Derivative
System Buffer YSI 2705
Calibrator Std YSI 2783 (5.00 g/L Lactose)
Linearity Std YSI 2784 (25.0 g/L Lactose)
Black Probe YSI 2702 Galactose Oxidase Membrane
White Probe n/a *
Sample Size 25 µL
Cal Station Station #1 Cal Well
Black Probe Parameters
Chemistry Lactose
Unit of Conc g/L
Cal value 5.00
End Point 30 sec
* If you have a dual channel unit, either Black or White, or both Black and White probes can be
configured for this chemistry.
Note: See Appendix A if concentration unit conversion is required.
Special Considerations:
»
If sample dilution is required, use reagent water.
»
The sample should be galactose-free. Galactose and other galactosides such as
raffinose and stachyose are substrates for galactose oxidase. They may interfere by
producing artificially high lactose readings.
»
The enzyme membrane integrity test involving the use of YSI 2363 Potassium
Ferrocyanide solution is not informative since ferrocyanide exists in the 2705 system
buffer.
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