4-7
$
This is a direct reading of sucrose in solution at the enzyme sensor. Three enzymes are co-
immobilized in the YSI Sucrose Membrane: Invertase, Mutarotase, and Glucose Oxidase.
Sucrose + H
2
O
Invertase
α-D-Glucose + (Fructose)
α-D-Glucose
Mutarotase
β-D-Glucose
β-D-Glucose + O
2
Glu Oxidase
H
2
O
2
+ D-Glucono-δ-Lactone
Through this chain of reactions the moles of hydrogen peroxide liberated is directly
proportional to the moles of sucrose.
System Buffer YSI 2357
Calibrator Std YSI 2780 (5.00 g/L Sucrose)
Linearity Std YSI 2778 (25.0 g/L Sucrose)
Black Probe YSI 2703 Sucrose Membrane
White Probe n/a *
Sample Size 25 µL
Cal Station Station #1 Cal Well
Black Probe Parameters
Chemistry Sucrose
Unit of Conc g/L
Cal value 5.00
End Point 30 sec
* If you have a dual channel unit, either Black or White, or both Black and White probes can be
configured for this chemistry.
Note: See Appendix A if concentration unit conversion is required.
Special Considerations:
»
If sample dilution is required, use reagent water or 2357 buffer.
»
The sample must be
dextrose-free
, or at least contain levels low enough not to
interfere with the sucrose reading. Since the sucrose membrane contains glucose
oxidase, dextrose will produce a probe signal. See Section 4.16 to measure dextrose
and sucrose simultaneously.
To Next Page
Loading...
