3-8
Ready to sample at Station #2
Pour a small amount of FCN Standard (1000 mg/dL) into a tube or cup and run it as a sample at
either the Manual or Test Tube Holder Station. The maximum allowable values for FCN
readings after calibrating with YSI standards are listed below.
Chemistry Membrane Calibration Standard FCN Limit
Dextrose 2365 2776, 2747 or 2355 0.05 g/L*
Ethanol 2768 2790 (@ 2.00 g/L) 0.05 g/L*
L-Glutamate 2754 2755 0.06 g/L*
L-Lactate 2329 2776, 2747 or 2327 0.03 g/L*
Sucrose 2703 2780 0.10 g/L*
Choline 2771 2772 0.02 g/L*
L-Glutamine 2735 2736 0.06 g/L*
Methanol 2725 2726 (@ 1.00 g/L) 0.05 g/L*
After a stable calibration with the recommended YSI calibration standard, FCN readings greater
than the limit may indicate membrane structural failure. Recalibrate and repeat the FCN test. If
readings are still high, refer to Section 8, Troubleshooting.
* If you use units other than g/L for the FCN test, refer to Appendix A for conversion values.
Linearity Test
Use the appropriate YSI linearity standard to test the linear range of the chemistry or
chemistries you have chosen. See the list below to be certain you are testing with the correct
standard solution.
Place your instrument in RUN Mode. When the unit is ready, the following display will appear.
Ready to sample at Station #2
Pour a small amount of linearity standard into a test tube or cup and run it as a sample at either
the Manual or Test Tube Holder Station. NOTE: If there is no test tube in the Test Tube Holder,
the sipper will automatically move to the Manual Station (#3) for aspiration.
Acceptable linearity values for YSI standards are ±5%. See the list of acceptable values below.
Chemistry Calibration Std Linearity Std Acceptable Range (g/L)*
Dextrose 2776 (2.50 g/L) 1531 (9.00 g/L) 8.55 to 9.45
Ethanol 2790 (2.00 g/L) 2790 (3.20 g/L) 3.04 to 3.36
Lactose 2783 (5.00 g/L) 2784 (25.00 g/L) 23.75 to 26.25
L-Glutamate 2755 (0.73 g/L) 2756 (1.46 g/L) 1.39 to 1.53
L-Lactate 2776 (0.50 g/L) 1530 (2.67 g/L) 2.54 to 2.80
Sucrose 2780 (5.00 g/L) 2778 (25.00 g/L) 23.75 to 26.25
Choline 2772 (0.175 g/L) 2773 (0.450 g/L) 0.43 to 0.47
L-Glutamine 2736 (0.73 g/L) 2737 (1.17 g/L) 1.11 to 1.23
Methanol 2726 (1.00 g/L) 2726 (2.50 g/L) 2.38 to 2.63
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