2 Installing GC Columns
36 5977B Series MSD Operation Manual
To Condition a Capillary Column
Materials needed
• Carrier gas, (99.9995% pure or better)
• Wrench, open-end, 1/4-inch and 5/16-inch (8710-0510)
Procedure
1 Install the column in the GC inlet. (See “To Install a Capillary Column in a
Split/Splitless Inlet” on page 33.)
2 Set a minimum velocity of 30 cm/s, or as recommended by the column
manufacturer. Allow the carrier gas to flow through the column at room
temperature for 15 to 30 minutes to remove air.
3 Program the oven from room temperature to the maximum temperature
limit for the column.
4 Increase the temperature at a rate of 10 to 15 °C/min.
5 Hold at the maximum temperature for 30 minutes.
6 Set the GC oven temperature to 30 °C and wait for the GC to become ready.
7 Attach the column to the GC interface. (See “To Install a Capillary Column
in the GC/MS Interface Using the Self-Tightening Column Nut” on page 37.)
WARNING
Do not condition your capillary column with hydrogen. Hydrogen accumulation in
the GC oven can result in an explosion. If you plan to use hydrogen as your carrier
gas, first condition the column with ultrapure (99.999% or better) inert gas such as
helium, nitrogen, or argon.
WARNING
The GC operates under high temperatures. To avoid burns, do not touch any GC parts
unless you are certain they are cool.
CAUTION
Never exceed the maximum column temperature, either in the GC/MS interface, the
GC oven, or the inlet.
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