2 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
Use the exercises in this guide to learn how to use the Agilent 6460 Triple
Quad Mass Spectrometer (Model K6460). You can do these exercises with the
demo data files, SulfaDrugs, shipped with the system (in the Data folder of
your Qualitative Analysis installation disk), or with data you acquire.
In Exercise 1, you learn how to determine the best acquisition settings for
analyzing your compounds of interest. These instructions help you understand
not only how to set up a worklist to optimize instrument parameters for best
sensitivity in acquisition, but also how to use the Agilent MassHunter
Qualitative Analysis program to identify parameter values producing optimum
signal response. You can also learn about the Qualitative Analysis program by
using the Qualitative Analysis Familiarization Guide or the Qualitative
Analysis online Help.
In Exercise 2, you learn how to use either an acquired data file or the
Quantitative Analysis report results to update a Dynamic MRM method. This
method allows you to easily set up a Dynamic MRM method.
In Exercise 3, you learn how to create a triggered Dynamic MRM method.
In Exercise 4, you learn how to use two programs to optimize parameters.
Agilent MassHunter Optimizer helps you optimize acquisition parameters.
Specifically, it automates the selection of the best precursor ion and the
fragmentor voltage for the most abundant precursor ion, selection of the best
product ions, and optimization of collision energy values for each transition
for a list of compounds you specify.
Each task is presented in a table with three columns:
• Steps – Use these general instructions to proceed on your own to explore
the program.
• Detailed Instructions – Use these if you need help or prefer to use a
step-by-step learning process.
• Comments – Read these to learn tips and additional information about each
step in the exercise.
NOTE
See the Concepts Guide to learn more about how the triple quadrupole mass spectrometer
works and why the fragmentor and collision energy voltages are important. For
background information, see Chapter 3, “Triple Quadrupole MS and Sensitivity”, in the
Concepts Guide. See the online Help for detailed information on how the program works.
To Next Page
Loading...
Need help?
General
