Exercise 4 – Optimize Acquisition parameters
Task 1. Use Optimizer to optimize acquisition parameters
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 59
Exercise 4 – Optimize Acquisition parameters
For this exercise you optimize a mixture of four sulfonamide compounds.
Task 1. Use Optimizer to optimize acquisition parameters
Optimizer helps you optimize acquisition parameters. Specifically, it
automates the selection of the best precursor ions, the optimization of the
fragmentor voltage for each precursor ion, selection of the best product ions,
and optimization of collision energy values for each transition for a list of
compounds you specify.
To do this task, you first need to create the method iiiSulfamix MRM_10.m in “Task
5. Find optimum collision energy for MRM acquisition”
on page 30. You do not need to
acquire the data file.
Steps Detailed Instructions Comments
1 Start the MassHunter Optimizer
program.
• Double-click the Optimizer icon. . • If you are optimizing peptides, use
the Optimizer for Peptides program.
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