Exercise 1 – Develop an acquisition method
Task 5. Find optimum collision energy for MRM acquisition
30 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
Task 5. Find optimum collision energy for MRM acquisition
In this task, you set up MRM acquisition methods for the sulfa drugs for
different collision energies. By examining the spectra and comparing peak
intensities, you determine the optimal collision energy settings for the
compounds.
Steps Detailed Instructions Comments
1 Set up three MRM acquisition
methods.
• Use all the MS parameters in the
example below except for the
collision energy value.
• Use collision energies of 10, 15
and 20.
• Save methods as iiiSulfamix
MRM_xx.m, where iii are your
initials and xx is the collision
energy.
a Click the QQQ tab.
b Set Scan Type to MRM.
c Enter all MS parameters shown in the
example below except for the collision
energy value.
d In the collision energy column, type
10 for each compound.
e Save the method as iiiSulfamix
MRM_10.m.
f Repeat step d and step e for collision
energies of 15, 20, 25, 30 and 35
saving the methods as iiiSulfamix
MRM_xx.m, where iii are your initials
and xx is the collision energy.
• Because the largest peaks were
produced with a collision energy of
15 in the previous exercise, you will
look at only those collision energies
to either side of 15.
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