Exercise 1 – Develop an acquisition method
Task 4. Determine product ion masses
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 29
6 Identify product ions.
• View each set of TICs and
spectra individually (e.g., 271
m/z first).
• Close the data files.
a In the Data Navigator, select the TICs
and spectra for the 271 m/z precursor
ion.
b Click the Show only the highlighted
items icon, .
c Click View > MS Spectrum Peak List 1.
d Examine the spectra to see which
fragment ions are produced at which
collision energies.
e Repeat steps a to d until all the
product ions are identified.
f Click the Close Data File icon in the
main toolbar, and click Close when the
dialog box containing the list of data
files pops up.
• The m/z 155.7 product ion is the
most abundant of any product ion
and the highest signal is recorded
at 15 V. This means that a good
choice for the MRM for
sulfamethizole would be 271.0 >
155.7 when the collision energy is
around 15 V.
• The peak may not be labeled if the
peak is too wide.
•
• The product ions appear to be:
Sulfamethizole-271.0 > 155.7
Sulfamethazine-279.0 > 185.7
Sulfachloropyridazine-285.0 > 155.7
Sulfadimethoxine-311.0 > 155.7
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