Exercise 3 – Create a Triggered Dynamic MRM acquisition method
Task 1. Create a Triggered Dynamic MRM method from a Dynamic MRM method manually
42 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
Exercise 3 – Create a Triggered Dynamic MRM acquisition
method
For this exercise you analyze a mixture of four sulfonamide compounds.
Task 1. Create a Triggered Dynamic MRM method from a Dynamic
MRM method manually
You can create a Triggered Dynamic MRM method directly from a Dynamic
MRM method. In a Triggered Dynamic MRM method, you specify some of the
transitions to be primary transitions. These transitions are acquired for the
entire retention time window. Some of these primary transitions are also
marked as triggers. As the data is acquired, the program checks whether or
not the abundances of the trigger transitions are higher than the threshold. If
the abundances are higher than the thresholds and other additional
conditions are met, then the secondary transitions are acquired. These other
conditions are described in the Concepts Guide.
Steps Detailed Instructions Comments
1 Open the method iiiSulfamix
dMRM2.m, where iii are your
initials.
a Click File > Open > Method.
b Select the iiiSulfamix_dMRM2.m
method.
c Click OK.
d Click Method > Save As.
e Type the new method name with the
format
iiiSulfamix_TriggeredDMRM.m.
f Click the Save button.
• A Triggered Dynamic MRM method
is a type of Dynamic MRM method.
The Scan Type for both methods is
Dynamic MRM.
• The Dynamic MRM method is the
template method for the
optimization.
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