Exercise 3 – Create a Triggered Dynamic MRM acquisition method
Task 1. Create a Triggered Dynamic MRM method from a Dynamic MRM method manually
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 43
2 Change the method to a triggered
dynamic MRM method.
a Click the Acquisition tab in the QQQ
tab in the Method Editor.
b Mark the Triggered check box in the
Triggered MRM section. This section
is only available if the Scan Type is
Dynamic MRM.
c Select whether to automatically mark
the highest product ion as the Primary.
d Enter the value for Repeats.
• Several columns are added to the
Scan segments table. These
columns only apply to a triggered
dynamic MRM method.
• The value Repeats is the number of
times to acquire each of the
secondary transitions when the
triggering conditions are met.
3 Add additional transitions. See the
next image.
a Select the first compound. Right-click
and click Insert Row. Repeat to insert
another row
b Modify the information in these rows
to match the information in the next
image.
c Repeat for each compound.
• For each compound, we are going
to add additional transitions.
4 Select the transitions that are the
Primary transitions. See the next
image.
a For each transition, mark the Primary
check box if it is a Primary transition.
b Verify that you have marked at least
one transition as the Primary
transition for each Compound Name.
• You can select multiple transitions
from each compound to be Primary
transitions. If a transition has the
same Compound Name, then it is
part of the same compound. You
must mark at least one transition as
a Primary transition for each
compound.
• Typically, the most abundant ion is
the Trigger transition.
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