Before you begin
Prepare to acquire data
4 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
Prepare to acquire data
If you do not intend to acquire data but want to learn how to use the
Qualitative Analysis program for method development, you can skip this step,
which tells you how to prepare the demo sample. You then do those tasks that
show you how to use the Qualitative Analysis program with the sulfa drug
data files shipped with the system.
Parts List The exercise in this guide uses this equipment and materials:
• Agilent K1260 Infinity LC modules: well-plate sampler, binary pump,
thermostatted column compartment, DAD
• Zorbax column (see Table 1 on page 4)
• A 1 ng/μL concentration of the sulfa mix sample (prepared in this step)
1 Prepare the LC solvents.
For the A channel, add 1 mL of 5M ammonium formate to a 1-liter reservoir
filled with HPLC-grade water.
For the B channel, add 1 mL of 5M ammonium formate to a 1-liter reservoir
filled with 90:10 acetonitrile and HPLC-grade water.
2 Prepare the sample.
a Add 10 μL of the sulfa mix from one of the ampoules (500 μL) to 990 μL
of solvent A in a 2 mL glass sample vial so that the final concentration is
1 ng/μL.
b Cap the vial and place in a sample location in the autosampler.
3 Set up the LC column.
Use the column from Table 1. Other columns and instrument parameters
may be used in these exercises, but some parameters may need adjustment,
and the results will differ.
Table 1 Zorbax column
Triple Quadrupole Column Description Particle
Size
Pore Size Part
Number
6460 RRHD Eclipse Plus
C18.2.1 mm x 50 mm
1.8 µm 95Å 959757-902
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