Exercise 1 – Develop an acquisition method
Task 3. Find optimum fragmentor voltage for maximum response
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 23
6 Select the fragmentor voltage that
produces the maximum response
for each of the precursor ions.
• Close the data files after you
determine the optimum voltage.
a In the Data Navigator window,
highlight the EICs for 271.0 m/z.
b Click the Show only the highlighted
items icon, .
Only the 271 m/z check boxes are
now marked.
c Look at the relative intensities of each
peak to determine which fragmentor
voltage setting will be best to use for
the 271 precursor.
d Repeat step a through step c for the
other three base peaks or precursor
ions.
e Click File > Close All.
f Click No in the Save dialog box.
• You press the Ctrl key to be able to
select multiple objects from the
Data Navigator window.
• You press the Shift key to be able to
select a group of objects.
• A fragmentor voltage of 100 should
be sufficient for each precursor ion.
• You can now determine the product
ions that are available for the
multiple-reaction monitoring
experiments to maximize sensitivity
for the analysis.
• You click Method > Save or
Method > Save As to save the
method changes.
• Click an EIC in the Data Navigator
window to change which chromatogram
is labeled in the Chromatogram Results
window. When the chromatogram label
color matches the color of the
chromatogram that has the highest
intensity, you use the Fragmentor
voltage that was used for that file.
Steps Detailed Instructions Comments
You can overlay the
chromatograms by
clicking the Overlaid
mode icon in the
Chromatogram Results
toolbar.
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