1290 Infinity Quaternary Pump User Manual 81
How to Optimize the Performance of Your Module
5
Operational Hints for the Multi Channel Gradient Valve (MCGV)
Operational Hints for the Multi Channel Gradient Valve (MCGV)
In a mixture of salt solutions and organic solvent the salt solution might
be well dissolved in the organic solvent without showing precipitations.
However in the mixing point of the gradient valve, at the boundary
between the two solvents, micro precipitation is possible. Gravity forces
the salt particles to fall down. Normally the A channel of the valve is used
for the aqueous/salt solution and the B channel of the pump is used for
the organic solvent. If used in this configuration the salt will fall back into
the aqueous solution and will be dissolved. When using the pump in a
different configuration (e.g., D - salt solution, A - organic solvent) the salt
can fall into the port of the organic solvent and may lead to performance
problems.
When using salt solutions and organic solvents it is recommended to connect the salt
solution to one of the bottom ports of the MCGV and the organic solvent to one of the upper
gradient valve ports. It is best to have the organic channel directly above the salt solution
channel. Regular flushing with water of all MCGV channels is recommended to remove all
possible salt deposits in the valve ports.
Precipitations formed during the mixing of buffers and organic solvents which do not
dissolve salts may cause a loss of pump performance (flow/retention time stability), a
blockage or internal leak of the pump. Avoid the use of such solvent combinations, as they
can cause irreproducible chromatographic results.
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