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Sample Introduction
Cleaning Syringes During an Autosampler Sequence
To ensure the syringe is properly cleaned between injections, rinse and fill each solvent
bottle with 4 mL of fresh solvent. The liquid level will be near the shoulder of the bottle.
Good laboratory practice dictates using no more than 2 mL of the 4 mL solvent for syringe
washes. The needle tip draws solvent 18.5 mm from the bottom of the vial.
Tapered Needle, 23-26s Gauge Autosampler Syringes
Volume
(µL)
Description Unit Needle Part No.
5 Tapered, fixed 23-26s/42/HP 5181-1273
Tapered, fixed 6/pk 23-26s/42/HP 5181-8810
Tapered, removable 23-26s/42/HP 5182-0835
Replacement needle for 5 µl syringe 3/pk 23-26s/42/HP 5182-0832
10 Tapered, fixed 23-26s/42/HP 5181-1267
Tapered, fixed 6/pk 23-26s/42/HP 5181-3360
Tapered, removable 23-26s/42/HP 5181-3321
Replacement needle for 10 µl syringe
3/pk 23-26s/42/HP 5181-3319
Tapered, fixed, PTFE-tipped plunger 23-26s/42/HP 5181-3354
Tapered, fixed, PTFE-tipped plunger 6/pk 23-26s/42/HP 5181-3361
Replacement plunger with PTFE tip
for fixed needle 10 µl syringe
5181-3365
Tapered, removable 23-26s/42/HP 5181-3356
Replacement plunger with PTFE tip
for removable needle 10
µl syringe
5181-3358
50 Tapered, fixed, PTFE-tipped plunger 23-26s/42/HP 5183-0314
100 Tapered, fixed, PTFE-tipped plunger
23-26s/42/HP 5183-2042
Tips & Tools
When cleaning syringes between
injections, use Agilent wash vials
(p/n 9301-0723) and diffusion
caps (p/n 07673-40180).
18.5 mm
4
mL fill volume
2.0 mL usable
s
olvent volume
Minimum solvent level
2.0 mL solvent
remains
N
eedle tip position when withdrawing solvent