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For optimum performance, ferrules should be replaced every time the column is replaced
and when performing column maintenance.
To minimize problems, follow these general techniques for ferrule installation:
• Don't overtighten – finger tighten the column nut, then use wrench to tighten.
• Maintain cleanliness.
• Bake out ferrules prior to use (Vespel and Vespel/Graphite only).
• Avoid contamination, such as fingerprint oils.
• Inspect used ferrules with magnifier for cracks, chips, or other damage before
reusing them.
• Change ferrules when new columns or injector/detector parts are installed.
Using the wrong ferrule or a worn-out ferrule to seal your column connection
can result in inconsistent and unreliable chromatography. An improper ferrule can cause
leaks which allow air and other contaminants to enter the instrument through the column
seal, causing major interference with column and detector performance.
Look for the following signals that indicate ferrule damage:
• Background noise from oxygen diffusing into the system
•
Column bleed catalyzed by oxygen
• Sample degradation
•
Sample loss
•
Increase in detector signal/noise
• Poor retention time reproducibility
T
ips & T
ools
Vespel/Graphite ferrules, 5181-3323
Ferrules
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