Awareness Technology CHROMATE - Adjusting Curves by Running Less than All the Calibrator

34 ChroMate
®
Microplate Reader Operator’s Manual Rev. Q
4.2.2.6 Adjusting curves by running less than all the Calibrator
CAUTION: Not all products are designed to
retain a stable standard curve.
Less than all the calibrators used in an assay can be run and the stored curve can be
adjusted accordingly. The adjustment factor will be calculated based on the average
percent change of all the new calibrators run compared to their stored absorbance
values. The new curve will be generated from the new calibrator(s) absorbance values
which are currently run and the adjusted absorbance values of the remaining calibrators
from the stored curve. This feature can be used to control changes in reagent activity
when using stored curves.
In Figure 4.2.2.6-1, Standard 5 is used to adjust the stored curve shown. Instead of
requesting an entirely new curve, Standard 5 is read individually.
Figure 4.2.2.6-1 Adjusting a curve with one or more Standards
By selecting this standard and all the stored standards, the new adjusted curve can be
activated.
Figure 4.2.2.6-2 Adjusted Curve