BÜCHI Labortechnik AG
www.buchi.com
6
2.4 MAKING ELECTRICAL AND FLUID
CONNECTIONS
1. Unpacking the Unit: Remove the ELSD from its shipping
container and position it on a level surface near the
column outlet of your HPLC system and the fume hood.
Make sure there is free flow of air to the bottom of the
ELSD and to the cooling fan at the back panel of the
ELSD. Allow the detector to warm to ambient temperature
if necessary. Save the shipping container for future use.
2. Exhaust Adapter:
Screw in the threaded exhaust
adapter provided in the accessory kit to the EXHAUST
port on the back panel of the unit.
3. Fill the Internal Drain Reservoir: Place a beaker under
the DRAIN port on the right side panel. Using a water
bottle or pipette, add water into the EXHAUST port on the
back panel of the unit until excess liquid is seen exiting
the DRAIN port. Filling the internal drain reservoir
prevents gas from escaping the drift tube.
4. Power Connection: Plug the power cord provided with
the unit into the power module on the back panel of the
detector. The module is autoselecting for 85-265V.
Only use the power supply cord
recommended by the
manufacturer.
5. 14-Pin Connector: Insert the 14-pin connector into the
port on the back panel. Make the appropriate wiring
connections to the 14-pin connector depending on which
of the following functions are needed:
Start: Pin 1: Ground (-) Pin 2: Signal (+)
Pins 1 and 2 on the Model 3300 ELSD can accept a
TTL/contact closure signal to start a method run on the
detector.
Standby: Pin 3: Ground (-) Pin 4: Signal (+)
Pins 3 and 4 on the Model 3300 ELSD can accept a
TTL/contact closure signal to put the detector into
Standby mode.
Gas Shutoff: Pin 5: Ground (-) Pin 6: Signal (+)
Pins 5 and 6 on the Model 3300 ELSD can accept a
TTL/contact closure signal to turn off gas flow at the end
of a run. This signal is typically sent from an autosampler
or a data collection system. Consult the appropriate
manuals for wiring information.
Autozero: Pin 7: Ground (-) Pin 8: Signal (+)
P
ins 7 and 8 on the Model 3300 ELSD can accept a
TTL/contact closure signal from a start signal cable to
autozero the detector. This signal is typically sent from an
autosampler or a manual injection valve with a position-
sensing switch. Consult the appropriate manuals for wiring
information.
External Output: Pin 9: Normally Open (NO) (+)
Pin 10: Common (-)
Pin 11: Normally Closed (NC) (+)
Pins 9, 10, and 11 on the Model 3300 ELSD can output a
TTL/contact closure signal to other instruments. Consult
the appropriate manuals for wiring details.
Fault Relay: Pin 12: Normally Open (NO) (+)
Pin 13: Common (-)
Pin 14: Normally Closed (NC) (+)
Pins 12, 13, and 14 on the Model 3300 ELSD can output
a TTL/contact closure signal to stop pump flow when an
error occurs on the detector. Consult the appropriate
manuals for wiring details.
6. Gas Connection: Connect the nitrogen gas supply
tubing to the GAS INLET on the back panel. The nitrogen
gas supply should be regulated from 65 – 80psig. A
stable gas flow and pressure are necessary for
reproducible results. The gas must be free of
contaminants, such as oil, water, particulates, or any other
non-volatile substances. A 0.1um gas filter is built into the
instrument.
7. Liquid Connection: Connect the column effluent line to
the LIQUID INLET with a 1/16" male fitting. The i.d. and
length of the tubing between the column and the detector
should be kept as small as possible to avoid band
broadening. 0.005" i.d. tubing is recommended for best
results. Tubing and fittings are included in the ELSD 3300
accessory kit.
8. Drain Setup: Attach the drain tubing and clamp included
with the detector to the DRAIN OUTLET on the right side
panel. Extend the tubing to a drain waste container (not
included) either at bench level if the detector is stacked or
at floor level. Make sure the container is sealed to prevent
solvent fumes from escaping. Monitor the liquid level in
the container during operation and decant excess liquid
when the level approaches the top of the container.
Do NOT allow the tubing to become
submerged in the liquid inside the
container. Remember that the waste
container will contain solvents from
your mobile phase and should be
disposed of properly.
9. Signal Output: Connect the signal cable provided with
the unit to the SIGNAL OUTPUT port on the back panel of
the detector and connect to your data collection device.
10. Exhaust Tubing: Connect the exhaust tubing included
with the unit to the EXHAUST outlet on the rear panel of
the unit. Extend the tubing to the hood/ventilation system.
There should be no low spots in the tubing where
condensate can collect.
Make sure the exhaust system provides adequate but
not excessive suction. Excessive suction can cause a
noisy baseline.
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