BÜCHI Labortechnik AG
www.buchi.com
1
1. INTRODUCTION
1.1 ABOUT THE MODEL 3300 ELSD
The Model 3300 ELSD is designed for use with High
Performance Liquid Chromatography (HPLC) systems to
analyze any sample compound that has sufficiently lower
volatility than the mobile phase. Some of its possible
application areas include carbohydrates, pharmaceuticals,
lipids, triglycerides, underivitized fatty and amino acids,
polymers, surfactants, nutraceuticals, and combinatorial
libraries.
Evaporative light scattering detection eliminates common
problems associated with other HPLC detectors. Refractive
Index (RI) detectors can be complicated by solvent front
interferences, baseline instability, and gradient
incompatibility. RI detectors can also have a less sensitive
response than ELSD. Low-wavelength UV can suffer
baseline drift with steep gradients and also requires that the
analyte contains a chromophore. ELSD does not have these
limitations. It can achieve stable baselines with multisolvent
gradients for improved resolution and faster separations.
Also, since ELSD response does not depend on the sample’s
optical characteristics, the sample does not require a
chromophore or fluorophore for detection.
The Model 3300 ELSD features the most advanced
evaporative light scattering detection technology available. It
provides sensitivity in the low nanogram range. An intuitive,
Windows
-based software interface provides a nested menu
o
f options for detector operation, including Method Wizard for
simplified method development, multiple languages, and built-
in Diagnostic tests for troubleshooting. The Model 3300 ELSD
also features compact, stackable dimensions, allowing it to fit
easily into limited laboratory space.
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