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Gatan K2 Summit - Camera Operation and System Setup; Temperature, Annealing, and Reference Images

Gatan K2 Summit
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K2 Summit and K2 Base Direct Detection Camera User's Guide
18
Setting Temperature
1. In the Camera menu, select Temperature and enter the set point value.
figure 1-9. Camera menu
Typical operating temperature is -20˚C.
2. If the camera chamber needs to be vented, set this to +20˚C. Wait for warm-up to
complete before venting.
The camera temperature is displayed in the Camera Monitor palette.
figure 1-10. K2 Sensor temperature
Anneal Cycle
Regular annealing (heating to +50˚C) of the sensor helps maintain top performance of the
sensor by reducing background levels and levels of surface contamination. Annealing can
also help to repair some radiation damage, although the camera will achieve the full radiation
damage specification if just a single anneal cycle is performed on the camera. We
recommend that an anneal cycle be performed each time the microscope does a cryo-cycle.
This can be done as often as every evening or less frequently (say once a week) during
extended periods of data collection.

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