K2 Summit and K2 Base Direct Detection Camera User's Guide
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3. Check the acquire and view settings, your preferred settings (Dark Subtraction for
instance) may be different between summit and base modes.
4. Switching between Summit and Base causes a temporary loss of temperature control, so
wait until the temperature is stable at the cold set point before proceeding with imaging.
5. When the sensor is cold, open the K2 toolbox and click Update Background Reference
to update the hardware dark reference.
6. If the camera does not function properly, follow the instructions above for powering
down and then powering up K2. If that fails, either repeat the power cycle or contact
Gatan for assistance.
Care of Detector
Temperature
Typical operating temperature is -20°C. Monitor the temperature; the Camera Monitor palette
indicates the camera temperature.
WARNING: Never operate the camera above +15°C, or with a vacuum above 1.5E-5 torr.
Check the flow of cooling water periodically. If the flow rate of the cooling water deviates
significantly from the value originally set (~15 liters/hour) make sure the lines are not
obstructed and adjust the pressure regulator to bring the flow back to the original level. If the
water flow stops while the Peltier cooler is on, damage to the camera may result.
WARNING: Ensure there are no air flow restrictions around PC, processor, TEC power
supply, and monitor.
Anneal Cycle
Regular annealing (heating to +50°C) of the sensor extends the usable life of the sensor and
minimizes contamination of the detector. If the camera is used a lot without warming, the
electron beam can harden any contaminants, making the CMOS Detector difficult to clean.
We recommend annealing cycle be performed each time the microscope undergoes a cryo-
cycle. Note it is not necessary to interrupt extended data-taking to perform the anneal cycle
however it is recommended to anneal the sensor about every 7 days.
To perform the Annealing cycle:
1. Select Camera > Temperature.
2. Select a set point of +50°C, click OK.
3. Click the Options button to select a duration other than 24 hours. Usually an annealing
cycle of overnight is sufficient.
4. Click the Start button to begin the cycle.
The camera cools down to +20°C after the annealing cycle is complete.
WARNING: DigitalMicrograph MUST CONTINUE RUNNING during the anneal cycle.
Auto Retraction
Delay setup (must be longer than longest intended image)
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