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5. ZERO and SPAN CALIBRATION:
Go to Operation, Reagents Setup, Install New Reagents menu, confirm the menu items and select
the “START NEW REAGENT CYCLE” function for the BioTector to prime the reagents and set the
Zero Adjust (zero offset) values automatically. See Section 2.2.2.1 Install New Reagents
and 8.2.5 New Reagents Program for details.
Observe that the automatic pressure/flow test passes when analyzer is started up. See Section
2.1.3 Analysis Data Screen and 8.3.4.5 Pressure/Flow Test Program for details.
It is recommended to check the zero response. When the Zero Calibration cycle is completed, go
to Operation, Start Stop menu (see Section 2.2.1 Start Stop for details) and stop the
analyzer. Go to Zero Calibration menu and select RUN ZERO CHECK function. Alternatively, to
confirm that the zero response is correct, connect DIW to the manual sample port and run 5
analysis cycles on DIW using the Manual Program menu. (If manual port is not available, use the input
point for SAMPLE 1. If the BioTector has been in storage for a long period, and if the zero readings are not
satisfactory, a second “Install New Reagents” cycle may be required.)
If the zero readings and CO
2
peaks are correct, items from 1 to 6 below can be skipped.
Confirm that the pH in the reactor is correct, using the test sequence in the pH Test menu. See
Section 8.1.1.5 pH Test for details.
Check for a pH of <2 during the TIC phase.
Check for a pH of >12 during the Base Oxidation phase.
Check for a pH of <2 during the TOC phase.
Run a further 2 reactions on DIW.
Run an “Install New Reagents” cycle on the system to adjust the zero offset.
Program the concentration of the available standard solution in the Span Calibration menu. (To
prepare a standard solution, see procedures described in Section 6.2 Calibration Standards or information
sheet “R009. Standard Solutions for BioTector Multi-component Analyzer”, which is available inside the
MMC/SD card shipped with the BioTector.)
Connect the standard solution to the MANUAL/CALIBRATION port. If these ports are not
available, use the SAMPLE 1 port. It is recommended that the standard solution should be located
at the same height as the sample chamber. Run the Span Calibration cycle using the RUN SPAN
CALIBRATION function in Span Calibration menu. A minimum of five complete analysis cycles is
recommended for the span calibration.
Download BioTector “All Data” in text format into the MMC/SD card using the SEND ALL DATA
function in Data Output menu to record all changes made in the system configuration. See Section
8.1.4 Data Output for details.
Go to Start Stop menu and start the BioTector. When the BioTector is running online, carefully
observe the first two or three reactions and confirm that the CO
2
peaks are correct.
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