Check for possible build-up of salts or any material at any of the PFA T fittings located
between the Ozone Generator and the Mixer Reactor. Clean the fittings and/or the tubing if
necessary.
Remove Ozone Line Filter and wash it with DIW (or tap water). Dry the filter well and reinstall
in place. See figure 1, table 2 in Section 4.1.1 Analysis Enclosure and figure 4 in Section
4.2.2 BioTector Sample Injection for details.
Using the Simulate menu (see Section 8.1.2 Simulate for details) and a 10ml graduated cylinder, check
and confirm that the pumps are pumping correctly.
Acid Pump rate for FMM20 Pump at 400 pulses should be between 3.80 ml and 4.20 ml.
(Depending on the quantity of the liquid injected into the reactor and due to an internal system interlock,
the system may request the activation of Reactor Purge cycle to purge any excess liquid from the
reactor. If necessary run “REACTOR PURGE” function in the Simulate menu.)
Base Pump rate for FMM20 Pump at 400 pulses should be between 3.80 ml and 4.20 ml.
Go to Zero Calibration menu and select RUN REAGENTS PURGE function to ensure that
acid and base tubes are full of reagents.
Important Note: For the correct operation of the system, the measured Acid and Base Pump rates must
be identical or similar. The maximum allowable difference in the measured volumes for acid and base
injections above should not be more than 0.2ml.
Sample Pump rate for WMM60 Pump at 16 pulses should be between 5.5ml and 7.5ml in ~8
seconds. (Any variation between these pumped volumes is corrected when the zero and span
calibration is carried out.)
Replace the 100mm filter mats in fan and vent housing (see item C4 in the service kit.)
Check the operation of the fan. (Note that at temperatures below 25°C, BioTector automatically
switches the fan off to stabilize system temperature using its own internal heat.)
Clean CO
2
analyzer optics by removing the 4x M4x60 Allen bolts on the detector section. If
necessary, clean both lenses, which are located on the detector and the source section of
the CO
2
Analyzer using the Lens Tissue (see item C5 in the service kits). Replace O-ring with
the 72-0325-30 O-ring provided (see item C6 in the service kit).
Oxygen Purity Test: Power up the system for at least 10 minutes before the oxygen purity
test is carried out. Using the Simulate menu (see Section 8.1.2 Simulate) set the MFC
(see figure 2 and table 2 in Section 4.1.1 Analysis Enclosure) flow to 10 l/h and flow
oxygen gas through the CO
2
analyzer for 5 minutes. At the end of this period, the CO
2
analyzer zero reading (ppm CO2) should be within ±2.5% of full scale of the CO
2
analyzer
range. For instance, if the CO
2
analyzer range is 2000ppm, then the CO
2
analyzer zero
reading should be typically within ±50ppm. Go to CO2 Analyzer menu (see section 8.3.10
CO2 Analyzer) to view the CO
2
Analyzer Range.
(If the CO
2
analyzer zero reading is outside the specifications, confirm that there is no CO
2
in the
oxygen gas by connecting the CO
2
filter “used with the base container” between the Cooler and CO
2
analyzer inlet port and set the MFC to 10 l/h. As the size of the CO
2
filter is small, keep the 10 l/h gas
flow running for at least for 5 minutes and record the CO
2
zero readings at the end of the 5 minute
period. If the CO
2
zero readings do not drop significantly with the CO
2
filter in place, this will indicate
that there is no CO
2
contamination in the oxygen supply.)
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