5. ZERO and SPAN CALIBRATION:
Connect DI Water to BioTector’s manual sample port and run 10 analysis cycles on DI Water
using the Manual Program menu to remove any TOC contamination in the system. (If MANUAL
port is not available, use SAMPLE 1 port, program the Sample Pump REVERSE time to 0 “zero”
seconds temporarily in Sample Pump menu, and start the analyzer using the Operation menu). If
the BioTector has been in storage for a long period, run additional analysis on DI Water until TOC
values become stable.
Go to Calibration, Zero Calibration menu, confirm the menu items and select the “RUN ZERO
CALIBRATION” function for the BioTector to set the Zero Adjust (zero offset) values automatically.
(If necessary ultra-pure water, which should not contain more than 5ppb TOC, can be used during
the Zero Calibration cycle to adjust the zero offset. To run Zero Calibration using ultra-pure water,
go to Maintenance, System Configuration, Sequence Program, Zero Program and set ZERO
WATER to YES. See ZERO WATER in section 8.3.4.2 Zero Program for details.)
Observe that the automatic pressure/flow test passes when analyzer is started up. See Section
2.1.3 Analysis Data Screen and 8.3.4.5 Pressure/Flow Test Program for details.
It is recommended to check the zero response. When the Zero Calibration cycle is completed, go
to Operation, Start Stop menu (see Section 2.2.1 Start Stop for details) and stop the
analyzer. Go to Zero Calibration menu and select RUN ZERO CHECK function. Alternatively, to
confirm that the zero response is correct, connect DIW to the manual sample port and run 5
analysis cycles on DIW using the Manual Program menu. (If manual port is not available, use the input
point for SAMPLE 1. If the BioTector has been in storage for a long period, and if the zero readings are not
satisfactory, a second “Install New Reagents” cycle may be required.)
If the zero readings and CO
2
peaks are correct, items from 1 to 6 below can be skipped.
Confirm that the pH in the reactor is correct, using the test sequence in the pH Test menu. See
Section 8.1.1.5 pH Test for details.
Check for a pH of <2 during the TIC phase.
Check for a pH of >12 during the Base Oxidation phase.
Check for a pH of <2 during the TOC phase.
Run a further 2 reactions on DIW.
Run an “Install New Reagents” cycle on the system to adjust the zero offset.
Program the concentration of the standard solution in the Span Calibration menu (2.3.2 Span
Calibration). The concentration of the calibration standard used must be typically greater than 50%
of the full scale of the RANGE the calibration is carried out. In order to see BioTector calibrated
ranges, see System Range Data screen (2.2.3 System Range Data Screen). (To prepare a
standard solution, see procedures described in Section 6.2 Calibration Standards or information sheet
“R009. Standard Solutions for BioTector Multi-component Analyzer”, which is available inside the MMC/SD
card shipped with the BioTector.)
Connect the standard solution to the MANUAL/CALIBRATION port. If these ports are not
available, use the SAMPLE 1 port. It is recommended that the standard solution should be located
at the same height as the sample chamber. Run the Span Calibration cycle using the RUN SPAN
CALIBRATION function in Span Calibration menu. A minimum of five complete analysis cycles is
recommended for the span calibration.
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