Copper
Copper
Copper_AVPP_Other_BIC_Eng_Ody.fm Page 5 of 6
above. Accept each standard additions reading by touching Read. Each addition should
reflect approximately 100% recovery.
8. After completing the sequence, touch Graph to view the best-fit line through
the standard additions data points, accounting for matrix interferences. Touch
View: Fit, then select Ideal Line and touch OK to view the relationship between
the sample spikes and the “Ideal Line” of 100% recovery.
See Section 3.2.2 Standard Additions on page 32 for more information.
Standard Solutions Method
Prepare a 4.00-mg/L Standard as follows:
1. Using Class A glassware, pipet 4.00 mL of Copper Standard Solution,
100-mg/L as Cu, into a 100-mL volumetric flask. Dilute to volume with
deionized water, stopper and invert to mix.
2. To adjust the calibration curve using the reading obtained with the
4.00-mg/L standard solution, touch
Options on the current program menu.
Touch
Standard Adjust.
3. Touch
On. Touch Adjust to accept the displayed concentration. If an alternate
concentration is used, touch the number in the box to enter the actual
concentration, then touch
OK. Touch Adjust.
See Section 3.2.4 Adjusting the Standard Curve on page 40 for more information.
Method Performance
Precision
Standard: 1.000 mg/L Cu
See Section 3.4.3 Precision on page 44 for more information, or if the standard
concentration did not fall within the specified range.
Sensitivity
See Section 3.4.5 Sensitivity on page 44 for more information.
Summary of Method
Copper in the sample reacts with a salt of bicinchoninic acid contained in
CuVer 1 or CuVer 2 Copper Reagent to form a purple colored complex in
proportion to the copper concentration. Test results are measured at 560 nm.
Program 95% Confidence Limits of Distribution
135 0.96–1.04 mg/L Cu
140 0.96–1.04mg/L Cu
Program Portion of Curve ∆Abs ∆Concentration
135 Entire range 0.010 0.04 mg/L Cu
140 Entire range 0.010 0.04 mg/L Cu
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