DR/2400
TPH (Total Petroleum Hydrocarbons)
TPHSoil_None_Other_IMM_Eng_Ody.fm Page 1 of 12
This TPH test can be used for both soil and water testing. When testing soil,
purchase the necessary items (see Required Apparatus on page 12) and perform the
Soil Extraction Procedure. When testing water samples only, proceed directly with
Immunoassay Procedure for Soil Extracts and Water Samples.The test requires about
20 to 30 minutes for complete analysis. As many as 10 cuvettes can be run
simultaneously.
• Read the entire procedure before starting. Identify and have ready all the necessary reagents, cuvettes, and other apparatus
before beginning the analysis. A 1-cm square cell holder is required.
• Timing is critical; follow instructions carefully.
• A consistent technique when mixing the cuvettes is critical to this test. The best results come from using the cuvette rack and
mixing as described in Using the 1-cm MicroCuvette Rack. Cuvettes can be mixed individually, but test results may not be as
consistent.
• Handle the cuvettes carefully. Scratches on the inside or outside may cause erroneous results. Carefully clean the outside of the
cuvettes with a clean absorbent cloth or tissue before placing them into the instrument.
• Antibody cuvettes and enzyme conjugate are made in matched lots. Do not mix reagent lots.
• Twenty Antibody Cuvettes are provided with each reagent set. One Antibody Cuvette will be used for each calibrator or sample.
Antibody Cuvettes are not reusable.
• To avoid damaging the Color Developing Solution, do not expose it to direct sunlight.
• Store the reagents at 4 °C (40 °F) when they are not in use. Allow the reagents to reach room temperature before using them in
an analysis. Actual testing may be done at temperatures ranging from 1–38 °C (34–100 °F).
• The Soil Extractant contains methyl alcohol which is poisonous and flammable. Before using this and other reagents, read the
Material Safety Data Sheet (MSDS) for proper use of protective equipment and other safety information.
• Hach Company recommends wearing protective nitrile gloves for this procedure.
TPH (Total Petroleum
Hydrocarbons)
Method 10050
Immunoassay Method*
* This test is semi-quantitative. Results are expressed as greater or less than the threshold value used.
Scope and Application: For soil and water
Tips and Techniques
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