DR/2400
Atrazine
ATZ_None_Other_IMM_Eng_Ody.fm Page 1 of 8
This method analyzes for Atrazine in water. Sample calibrators and reagents are
added to cuvettes coated with Atrazine-specific antibodies. The color that
develops is then measured and compared with the color measurements of the
calibrators. The test requires about 30 minutes for complete analysis. As many as
20 cuvettes (18 samples and 2 calibrators) can be run simultaneously.
• Read the entire procedure before starting. Identify and have ready all the necessary reagents, cuvettes, and other apparatus
before beginning the analysis. A 1-cm square cell holder is required for this procedure.
• Timing is critical; follow instructions carefully.
• A consistent technique when mixing the cuvettes is critical to this test. The best results come from using the cuvette rack and
mixing as described in Using the 1-cm MicroCuvette Rack. Cuvettes can be mixed individually, but test results may not be as
consistent.
• Handle the cuvettes carefully. Scratches on the inside or outside may cause erroneous results. Carefully clean the outside of the
cuvettes with a clean absorbent cloth or tissue before placing them into the instrument.
• Antibody cuvettes and enzyme conjugate are made in matched lots. Do not mix reagent lots.
• To avoid damaging the Color Developing Solution, do not expose it to direct sunlight.
• The cuvette rack is designed to be inverted with the cuvettes in place. This is especially helpful when running many samples at
once; the cuvettes can remain in the rack and be processed together until they are read in the spectrophotometer.
• Twenty Antibody Cuvettes are provided with each reagent set. One Antibody Cuvette will be used for each calibrator and each
sample. Cuvettes are not reusable.
• Hach Company recommends wearing protective nitrile gloves for this procedure.
Atrazine
Method 10050
Immunoassay Method*
* This test is semi-quantitative. Results are expressed as greater or less than the threshold value used.
Scope and Application: For water
Tips and Techniques
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