DR/2400
Surfactants, Anionic (Detergents)
Detergents_None_Other_CVI_Eng_Ody.fm Page 1 of 4
• Use benzene only in a well-ventilated area.
• To prevent water droplets from forming in the sample cells, use only dry sample cells and discard the first few mL of benzene.
Additionally, it helps to transfer the liquid from the funnel to a sample cell, let it sit for a few seconds, and decant to a second cell
for reading.
• Excessive agitation may cause an emulsion to form, which in turn makes the phases separate more slowly. Should this occur,
remove most of the water layer, then gently agitate the contents of the funnel with a clean Teflon
®
-coated rod or other such inert
tool.
• Spilled reagent will affect test accuracy and is hazardous to the skin and other materials.
• Acetone (Cat. No. 14429-49) may be used to clean benzene from glassware.
• Benzene (D018) solutions are regulated as hazardous waste by the Federal RCRA. Do not pour these materials down the drain.
Collect water saturated with benzene and benzene solutions for disposal with laboratory solvent wastes. See Section 4 for more
information on proper disposal of these materials.
• For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized
water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank adjust. See the
instrument manual for more information on Running a Reagent Blank.
Surfactants, Anionic
(Detergents)
Method 8028
Crystal Violet Method*
* Analytical Chemistry, 38, 791 (1966).
(0.002 to 0.275 mg/L as LAS)
Scope and Application: For water, wastewater, and seawater
1. Touch
Hach Programs.
Select program
710 Surfactants.
Touch
Start.
2. Fill a clean 500-mL
graduated cylinder to the
300 mL mark with
sample. Pour the sample
into a clean 500-mL
separatory funnel.
3. Add 10 mL of Sulfate
Buffer Solution. Stopper
the funnel. Shake the
funnel for five seconds.
4. Add the contents of
one Detergents Reagent
Powder Pillow to the
funnel. Stopper the
funnel and shake until
the powder dissolves
completely. (This may
take some time.)
Tips and Techniques
10 mL
20 mL
25 mL
Method 8028
Hach Programs
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