DR/2400
Phosphorus, Reactive (Orthophosphate)
PhosphorusReac_None_Other_AMI_Eng_Ody.fm Page 1 of 6
• For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized
water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank adjust. See
Section 2.6.2 Reagent Blank on page 20.
• Substitute the contents of one Amino Acid Reagent Powder Pillow (Cat. No. 804-99) for 1 mL of amino acid reagent solution in
step 4, if desired.
Phosphorus, Reactive
(Orthophosphate)
Method 8178
Amino Acid Method*
*Adapted from Standard Methods for the Examination of Water and Wastewater
(0.23 to 30.00 mg/L PO
4
3–
)
Scope and Application: For water, wastewater, and seawater
1. Touch
Hach Programs.
Select program
485 P React. Amino.
Touch
Start.
2. Fill a 25-mL mixing
cylinder with 25 mL of
sample.
3. Add 1 mL of
Molybdate Reagent using
a 1-mL calibrated
dropper.
4. Add 1 mL of Amino
Acid Reagent Solution.
Stopper and invert
several times to mix (this
is the prepared sample).
A blue color will form if
phosphate is present.
Tips and Techniques
10 mL
20 mL
25 mL
Method 8178
Hach Programs
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