Hach DR2400 - Page 782

TPH (Total Petroleum Hydrocarbons)
© Hach Company, 2003. All rights reserved. Printed in the U.S.A. 3/04 1ed
Page 12 of 12 TPHSoil_None_Other_IMM_Eng_Ody.fm
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After incubation, the sample and unbound enzyme conjugate are washed from
the cuvette and a color-development reagent is added. The enzyme in the
conjugate catalyzes the development of color. Therefore, there is an inverse
relationship between color intensity and the amount of TPH in the sample. The
resulting color is then compared with a calibrator to determine whether the TPH
concentration in the sample is greater or less than the threshold levels. The TPH
concentration is inversely proportional to the color development: the lighter the
color, the higher the TPH concentration. Test results are measured at 450 nm.
Required Reagents
Description Unit Cat. No.
Deionized water.................................................................................................................. 500 mL...................272-48
Reagent Set, TPH
*
............................................................................................................... 20 cuvettes ........27743-00
Required Apparatus
Cell holder, 1-cm square .................................................................................................... each....................59065-00
Caps, flip spout ................................................................................................................... 2/pkg.................25818-02
Marker, laboratory.............................................................................................................. each....................20920-00
Rack, for 1-cm Micro Cuvettes.......................................................................................... each....................48799-00
TenSette, Pipet, 0.1–1.0mL ................................................................................................. each....................19700-01
Tips, for pipettor 19700-01................................................................................................. 1000/pkg...........21856-28
Wipes, disposable ............................................................................................................... box .....................20970-00
For Soil Extraction only:
Soil Scoop, 5-g, 4.25-cc ....................................................................................................... each....................26572-05
Soil Extraction Refill Kit .................................................................................................... each....................27752-00
Includes:
Dropper, LDPE, 0.5 and 1.0-mL ................................................................................. 20/pkg...............21247-20
Filter and Barrel Assembly ......................................................................................... 20/pkg...............25676-20
Sodium Sulfate, anhydrous ........................................................................................ 250 g.....................7099-14
Soil Extractant Solution............................................................................................... 200 mL...............25677-29
Soil Sample Container ................................................................................................. 20/pkg...............25929-20
Spatula, disposable ...................................................................................................... 2/pkg.................25693-20
Weighing Boat, 8.9-cm square .................................................................................... 20/pkg...............21790-20
* Immunoassay components are manufactured for Hach Company by Beacon Analytical Systems, Inc.