Metolachlor
Metolachlor
MET_None_Other_IMM_Eng_Ody.fm Page 7 of 8
Summary of Method
Hach immunoassay tests use antigen/antibody reactions to test for specific
organic compounds in water and soil. Metolachlor-specific antibodies, attached
to the walls of plastic cuvettes, selectively bind and remove Metolachlor from
complex sample matrices. A prepared sample and a reagent containing enzyme-
conjugate molecules (analyte molecules attached to molecules of an enzyme) are
added to the Antibody Cuvettes. During incubation, enzyme-conjugate
molecules and Metolachlor compete for binding sites on the antibodies. Samples
with higher levels of analyte will have more antibody sites occupied by
Metolachlor and fewer antibody sites occupied by the enzyme-conjugate
molecules.
After incubation, the sample and unbound enzyme conjugate are washed from
the cuvette and a color-development reagent is added. The enzyme in the
conjugate catalyzes the development of color. Therefore, there is an inverse
relationship between color intensity and the amount of Metolachlor in the
sample. The resulting color is then compared with a calibrator to determine
whether the Metolachlor concentration in the sample is greater or less than the
threshold levels.
Test results are measured at 450 nm.
Required Reagents
Description Unit Cat. No.
Reagent Set, Metolachlor
*
.................................................................................................. 20 cuvettes ........28135-00
Required Apparatus
Adapter, 1-cm square cell .................................................................................................. each....................59459-00
Caps, flip spout ................................................................................................................... 2/pkg.................25818-02
Cell holder, 1-cm square .................................................................................................... each....................59065-00
Marker, laboratory.............................................................................................................. each....................20920-00
Rack, for 1-cm Micro Cuvettes.......................................................................................... each....................48799-00
Wipes, disposable ............................................................................................................... box .....................20970-00
TenSette
®
, Pipet, 0.1–1.0 mL.............................................................................................. each....................19700-01
Tips, for pipettor 19700-01................................................................................................. 1000/pkg...........21856-28
* Immunoassay components are manufactured for Hach Company by Beacon Analytical Systems, Inc.
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