Nickel
Nickel
Page 2 of 6
Nickel_PP_Other_HEP_Eng_Ody.fm
5. When the timer
beeps, add the contents of
one Nickel 2 Reagent
Powder Pillow to the
funnel. Stopper and
invert to mix.
6. Touch the timer icon.
Touch
OK.
A second five-minute
reaction period will begin.
7. When the timer
beeps, add 10 mL of
chloroform. Stopper and
invert gently. With the
funnel inverted and the
tip pointed away from
people, open the
stopcock to vent.
8. Close the stopcock
and invert for 30 seconds.
9. Touch the timer icon.
Touch
OK.
A third five-minute
reaction period will begin.
Invert the funnel several
times over the five minute
period.
10. When the timer
beeps, wait for the layers
to separate. Insert a
pea-sized cotton plug
into the delivery tube of
the funnel. Remove the
stopper and drain the
chloroform layer (bottom
layer) into a sample cell.
(This is the prepared
sample.)
Stopper the funnel.
11. Repeat steps 7
through 10 two
additional times with
10-mL volumes of
chloroform. The
five-minute reaction
period is not necessary.
Stopper the funnel and
invert to mix. Wait for the
layers to separate, and
continue.
12. Cap the sample cell
and invert to mix the
extracts. The final volume
will be about 25 mL due
to the slight solubility of
chloroform in water.
HRS MIN SECHRS MIN SEC
10 mL
20 mL
25 mL
Repeat
Steps 7—10
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