Nitrate
© Hach Company, 2003. All rights reserved. Printed in the U.S.A. 3/04 1ed
Page 4 of 4 Nitrate_TNT_High_CHA_Eng_Ody.fm
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Method Performance
Precision
Standard: 10.0 mg/L NO
3
–
–N
See Section 3.4.3 Precision on page 44 for more information, or if the standard
concentration did not fall within the specified range.
Sensitivity
See for more information.
Summary of Method
Nitrate in the sample reacts with chromotropic acid under strongly acidic
conditions to yield a yellow product with a maximum absorbance at 410 nm.
Required Reagents
Quantity Required
Description Per Test Unit Cat. No.
Test ‘N Tube NitraVer
®
X Nitrate Reagent Set (50 tests)............................................................................26053-45
Required Apparatus
Adapter, 16-mm Cell ................................................................................1 ....................... each....................59457-00
Funnel, micro, poly...................................................................................1 ....................... each....................25843-35
Pipet, TenSette
®
, 0.1 to 1.0 mL ................................................................1 ....................... each....................19700-01
Pipet Tips, for 19700-01 TenSette
®
Pipet ...............................................varies .............. 50/pkg...............21856-96
Sample Cells, 10-mL, w/cap...................................................................2 ....................... 6/pkg.................24276-06
Test Tube Rack, cooling............................................................................1–3 ................... each....................18641-00
Required Standards
Nitrate Nitrogen Standard Solution, 10-mg/L N .......................................................... 500 mL...................307-49
Nitrate Nitrogen Standard Solution, Voluette
®
Ampule, 500-mg/L N...................... 16/pkg...............14260-10
Water, deionized ................................................................................................................. 4 liters ....................272-56
Program 95% Confidence Limits of Distribution
344 9.5–10.5 mg/L NO
3
–
–N
Portion of Curve ∆Abs ∆Concentration
Entire range 0.010 0.2 mg/L NO
3
–
–N