Nitrogen, Total
Nitrogen, Total
Page 2 of 6
NitrogenTot_TNT_High_PSD_Eng_Ody.fm
5. Place the vials in the
reactor. Heat for exactly
30 minutes.
6. Using finger cots,
immediately remove the
hot vials from the reactor.
Cool the vials to room
temperature.
7. Touch
Hach Programs.
Select program
395 N, Total HR TNT.
Touch
Start.
8. Remove the caps
from the digested vials
and add the contents of
one Total Nitrogen (TN)
Reagent A Powder Pillow
to each vial.
9. Cap the tubes and
shake for 15 seconds.
10. Touch the timer icon.
Touch
OK.
A three-minute reaction
period will begin.
11. After the timer beeps,
remove the caps from the
vials and add one TN
Reagent B Powder Pillow
to each vial.
12. Cap the tubes and
shake for 15 seconds.
Note: The reagent will not
completely dissolve. This will
not affect accuracy.
The solution will begin to
turn yellow.
13. Touch the timer icon.
Touch
OK.
A two-minute reaction
period will begin.
14. After the timer beeps,
remove the caps from
two TN Reagent C vials
and add 2 mL of
digested, treated sample
to one vial. Add 2 mL of
digested, treated reagent
blank to the second TN
Reagent C vial.
15. Cap the vials and
invert ten times to mix.
Use slow, deliberate
inversions for complete
recovery.
The tubes will be warm to
the touch.
16. Touch the timer icon.
Touch
OK.
A five-minute reaction
period will begin.
The yellow color will
intensify.
Hach Programs
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