Hach DR2400 - Page 583

PCB (Polychlorinated Biphenyls)
PCB (Polychlorinated Biphenyls)
PCB_None_Other_IMM_Eng_Ody.fm Page 3 of 10
1. Touch
Single Wavelength
then touch the λ button.
Type in 450 nm and
touch
OK.
Note: Use the 1-cm square cell
holder for this procedure.
2. Label an Antibody
Cuvette for each
calibrator and each
sample to be tested.
Note: As many as 10 cuvettes
may be tested at one time and
may comprise any combination
of samples and calibrators.
3. Place the cuvettes
snugly into the rack.
4. Pipet 0.5 mL of
Diluent Solution into
each cuvette.
Note: The same pipette tip can
be used repeatedly for this
step.
Note: Have the necessary
apparatus at hand for the next
four steps as they must be
done without delay.
5. Use a Wiretrol
®
pipet
to transfer the
appropriate volume of
calibrator or sample
extract into each cuvette.
Note: When testing at the
1 ppm and/or 5 ppm levels, use
50 µL of calibrator and sample.
When testing at the 10 ppm
and/or 50 ppm levels, use
10 µL of calibrator and sample.
Note: Use a separate capillary
tube for each solution.
6. Immediately pipet
0.5 mL of PCB Enzyme
Conjugate into each
calibrator and
sample cuvette.
Note: The same pipette tip can
be used repeatedly for this
step.
7. Touch the timer icon.
Enter 10 minutes and
touch
OK.
A 10-minute reaction time
will begin. Proceed
immediately to the next
step.
8. Mix the contents of
the cuvettes for
30 seconds using the
technique described in
Using the 1-cm
MicroCuvette Rack on
page 7.
Immunoassay Procedure for Soil Extracts
λ
OK
54 0
HRS MIN SECHRS MIN SEC