Hach DR2400 - Page 590

PCB (Polychlorinated Biphenyls)
© Hach Company, 2003. All rights reserved. Printed in the U.S.A. 3/04 1ed
Page 10 of 10 PCB_None_Other_IMM_Eng_Ody.fm
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Telephone: (970) 669-3050
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Call 800-227-4224
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Summary of Method
Hach immunoassay tests use antigen/antibody reactions to test for specific
organic compounds in water and soil. Antibodies specific for PCB are attached
to the walls of plastic cuvettes. They selectively bind and remove PCB from
complex sample matrices. A prepared sample and a reagent containing enzyme-
conjugate molecules (analyte molecules attached to molecules of an enzyme) are
added to the Antibody Cuvettes. During incubation, enzyme-conjugate
molecules and PCB compete for binding sites on the antibodies. Samples with
higher levels of analyte will have more antibody sites occupied by PCB and
fewer antibody sites occupied by the enzyme-conjugate molecules.
After incubation, the sample and unbound enzyme conjugate are washed from
the cuvette and a color-development reagent is added. The enzyme in the
conjugate catalyzes the development of color. Therefore, there is an inverse
relationship between color intensity and the amount of PCB in the sample. The
resulting color is then compared with a calibrator to determine whether the PCB
concentration in the sample is greater or less than the threshold levels. The PCB
concentration is inversely proportional to the color development: the lighter the
color, the higher the PCB concentration. Test results are measured at 450 nm.
Required Reagents
Description Unit Cat. No.
Reagent Set, PCB
*
............................................................................................................... 20 cuvettes ........27735-00
Deionized water.................................................................................................................. 500 mL...................272-48
Required Apparatus
Cell holder, 1-cm square .................................................................................................... each....................59065-00
Caps, flip spout ................................................................................................................... 2/pkg.................25818-02
Marker, laboratory.............................................................................................................. each....................20920-00
Rack, for 1-cm Micro Cuvettes.......................................................................................... each....................48799-00
TenSette
®
, Pipet, 0.1–1.0 mL.............................................................................................. each....................19700-01
Tips, for TenSette
®
, Pipet 19000-01................................................................................... 1000/pkg...........21856-28
Wipes, disposable ............................................................................................................... box .....................20970-00
For Soil Extraction only:
Soil Scoop, 5-g, 4.25-cc ....................................................................................................... each....................26572-05
Soil Extraction Refill Kit .................................................................................................... each....................27752-00
Includes:
Dropper, LDPE, 0.5 and 1.0-mL ................................................................................. 20/pkg...............21247-20
Filter and Barrel Assembly ......................................................................................... 20/pkg...............25676-20
Sodium Sulfate, anhydrous ........................................................................................ 250 g.....................7099-29
Soil Extractant Solution............................................................................................... 200 mL...............25677-29
Soil Sample Container ................................................................................................. 20/pkg...............25929-20
Weighing Boat, 8.9-cm square .................................................................................... 20/pkg...............21790-20
Spatula, disposable ...................................................................................................... 2/pkg.................25693-20
* Immunoassay components are manufactured for Hach Company by Beacon Analytical Systems, Inc.