Hach DR2400 - Page 592

Phenols
Phenols
Page 2 of 6
Phenols_None_Other_4AM_Eng_Ody.fm
5. Pour the measured
sample into another
500-mL separatory funnel
(this is the prepared
sample).
6. Add 5 mL of
Hardness Buffer to each
separatory funnel.
Stopper and shake to
mix.
7. Add the contents of
one Phenol Reagent
Powder Pillow to each
separatory funnel.
Stopper and shake to
dissolve.
8. Add the contents of
one Phenol 2 Reagent
Powder Pillow to each
separatory funnel.
Stopper and shake to
dissolve.
9. Add 30 mL of
chloroform to each
separatory funnel.
Stopper each funnel.
10. Invert each funnel
and temporarily vent.
Shake each funnel briefly
and vent. Then
vigorously shake each
funnel for a total of
30 seconds (venting if
necessary).
11. Remove the stoppers.
Allow both funnels to
stand until the
chloroform settles to the
bottom of the funnel.
The chloroform layer will
be yellow to amber if
phenol is present.
12. Insert a large,
pea-sized cotton plug
into the delivery tube of
each funnel.
Filtering the chloroform
layer through the cotton
removes suspended water
or particles. The volume
of chloroform extract will
be about 25 mL.
S B S
B S
B S