Phosphorus, Reactive (Orthophosphate)
Phosphorus, Reactive (Orthophosphate)
Page 4 of 6
PhosphorusReac_None_Other_AMI_Eng_Ody.fm
3. Touch OK to accept the default values for standard concentration, sample
volume, and spike volumes. Touch
Edit to change these values. After values
are accepted, the unspiked sample reading will appear in the top row. See
Section 3.2.2 for more information.
4. Snap the neck off a Phosphate 2-mL Ampule Standard, 500-mg/L PO
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.
5. Prepare three sample spikes. Fill three mixing cylinders (Cat. No. 1896-40)
with 25 mL of sample. Use the TenSette
®
Pipet to add 0.1 mL, 0.2 mL, and
0.3 mL of standard, respectively, to each sample and mix thoroughly.
6. Analyze each sample spike as described in the procedure above, starting with
the 0.1 mL sample spike. Accept each standard additions reading by touching
Read. Each addition should reflect approximately 100% recovery.
7. After completing the sequence, touch
Graph to view the best-fit line through
the standard additions data points, accounting for matrix interferences. Touch
View: Fit, then select Ideal Line and touch OK to view the relationship between
the sample spikes and the “Ideal Line” of 100% recovery.
See Section 3.2.2 Standard Additions on page 32 for more information.
Standard Solution Method
1. Use a 10-mg/L Phosphate Standard Solution. Perform the amino acid
procedure as described above.
2. To adjust the calibration curve using the reading obtained with the
10-mg/L PO
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Phosphate Standard Solution, touch Options on the current
program menu. Touch
Standard Adjust.
3. Touch
On. Touch Adjust to accept the displayed concentration (the value
depends on the selected chemical form). If an alternate concentration is used,
touch the number in the box to enter the actual concentration, then touch
OK.
Touch
Adjust.
Method Performance
Precision
Standard: 10.00 mg/L PO
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See Section 3.4.3 Precision on page 44 for more information, or if the standard
concentration did not fall within the specified range.
Sensitivity
See Section 3.4.5 Sensitivity on page 44 for more information.
Program 95% Confidence Limits of Distribution
485 9.86–10.14 mg/L PO
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Portion of Curve ∆Abs ∆Concentration
Entire range 0.010 0.23 mg/LPO
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