Phosphorus, Reactive (Orthophosphate)
© Hach Company, 2003. All rights reserved. Printed in the U.S.A. 3/04 1ed
Page 4 of 4 PhosphorusReac_TNT_High_MOL_Eng_Ody.fm
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Method Performance
Precision
Standard: 50.0 mg/L PO
4
3–
See Section 3.4.3 Precision on page 44 for more information, or if the standard
concentration did not fall within the specified range.
Sensitivity
See Section 3.4.5 Sensitivity on page 44 for more information.
Summary of Method
Orthophosphate reacts with molybdate in an acid medium to produce a mixed
phosphate/molybdate complex. In the presence of vanadium, yellow
molybdovanadophosphoric acid forms. The intensity of the yellow color is
proportional to the phosphate concentration. Test results are measured at
420 nm.
Required Reagents
Quantity Required
Description Per Test Unit Cat. No.
High Range Reactive Phosphorus Test ’N Tube™ Reagent Set................................... 50 vials...............27673-45
Includes:
(1) Reactive High Range Phosphorus Test ’N Tube Vials.............1 ....................... 50/pkg
....................................*
(2) Water, deionized ...........................................................................5 mL ................ 100 mL...................272-42
Required Apparatus
Adapter, 16-mm Cell ................................................................................1 ....................... each....................59457-00
Dropper, LDPE, 0.5–1.0 mL.....................................................................1 ....................... 20/pkg...............21247-20
Pipet, TenSette
®
, 1 to 10 mL ....................................................................1 ....................... each....................19700-10
Pipet Tips, for 19700-10 TenSette
®
Pipet ...............................................1 ....................... 50/pkg...............21997-96
Test Tube Rack...........................................................................................1–3 ................... each....................18641-00
Required Standards
Phosphate Standard Solution, 50-mg/L, as PO
4
3–
........................................................ 500 mL...................171-49
Phosphate Standard Solution, Voluette
®
ampule,
500-mg/L as PO
4
3–
, 10-mL................................................................................................ 16/pkg...............14242-10
Program 95% Confidence Limits of Distribution
540 48.7–51.3 mg/L PO
4
3–
Portion of Curve ∆Abs ∆Concentration
Entire range 0.010 1.0 mg/L PO
4
3–
* Not available separately.